From HoNwiki

Runes are power-ups which spawn every 2 minutes after 2 minute mark, this means they spawn at 02:00, 04:00, 06:00 and so on.

There are 5 types of runes which can be picked up normally or picked up with a Bottle, if you bottle the rune, you have 2 minutes to use it before it uses itself automatically. Upon consuming the rune, the bottle will be filled.

Types of Runes

Double Damage

Double Damage runes increase your damage by 100% of your base damage for 45 seconds.

Illusion

When used it spawns two Illusions which last for 75 seconds.

Haste

When used it increases your movement speed to the maximum of 522 for 30 seconds, during which time slow effects are ignored.

Invisibility

When used it applies invisibility with 2 seconds fade time for 60 seconds or until broken. During those first 2 seconds you may perform any action without breaking the invisibility.

Regeneration

When used it gives you 6% health regeneration per second and 10% mana regeneration per second for 30 seconds. The effect ends if you take damage or reach full health and mana.