I thought I would share my thesis project with all of you.
In short the thesis is about sequence mining and visualization of sequence graphs to generate flow maps. You can consider flow maps an advanced version of heat maps, where the focus is on analyzing movement (instead of generating a static snapshot).
The following conclusion can be derived from the video and series of images below:
Players use bottle charges in relation to getting the rune and most activity with the Bottle is centered around the rune spawns.
From the last image it is shown that roughly half of the players use the bottom rune to gank bot, and the other half go back mid and continue farming (when playing as Soul Stealer).